2006/02/22 We are very happy to announce the publications about ASKA library and Keio collection, respectively.
1) ASKA library
Kitagawa, M., Ara, T., Arifuzzaman, M., Ioka-Nakamichi, T., Inamoto, E., Toyonaga, H. and Mori, H. Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12, 291-299 (2005). http://dnaresearch.oxfordjournals.org\ /current.dtl
2) Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K.A., Tomita, M., Wanner, B.L. and Mori, H. Construction of Escherichia coli K-12 in-frame, single-gene knock-out mutants -- the Keio collection. Mol Systems Biol, doi:10.1038/msb4100050 (2006). http://www.nature.com/msb/index.html
You would be able to download PDF files of the manuscripts and the supplementary information from each web-site.
2005/02/08 This is a News Letter providing by GenoBase management group (http://ecoli.naist.jp) in Nara Institute of Science and Technology.
We will issue news concerned about Escherichia coli Resources we made, ASKA library (series of ORF plasmid clone library) amd Keio collection (gene Knock Out strain library) and related articles. Also we would like to provide spaces to researchers using E. coli for discussion or announcements.

We are very happy to announce the half way completion of re- construction of revised E. coli ORF clone based on new annotation fused with GFP. As previously reported in News Letter circularized on Nov. 1, 2004, about 950 target ORFs were required to be re- constructed and now about 600 out of those are completed and ready for distribution. Remaining targets are now under confirmation by sequencing and will be prepared for distribution soon. Few are still under construction.A list of genes started distribution or under reconstruction is now available from our web page (archive.html).
If you had previously requested our clones but we could not send them for this reason, please confirm if your gene have released and request them again from our web form (ResourceManage.jsp).
-- Members in this mailing list were registered from the list of participants of previous E. coli symposium or workshop held in Japan, we organized, and users of our resources. If you don't want to receive this message, please send email to ecoli_admin@gtc.naist.jp with leave in subject field.
2004/12/07 This is a News Letter providing by GenoBase management group (http://ecoli.naist.jp) in Nara Institute of Science and Technology.
We will issue news concerned about Escherichia coli Resources we made, ASKA library (series of ORF plasmid clone library) amd Keio collection (gene Knock Out strain library) and related articles. Also we would like to provide spaces to researchers using E. coli for discussion or announcements.

We must apologize to make you confused by receiving mails not anticipated. This was our fault not to inform you about mailing list of ecoli@gtc.naist.jp.
Members in this mailing list were registered from the list of participants of previous E. coli symposium or workshop held in Japan, we organized, and users of our resources. If you donEt want to receive this message, please send email to ecoli_admin@gtc.naist.jp with leave in subject field.

(1) update information of GenoBase, E. coli genome database ORF annotation table ver. 1.2 -> ver. 1.3
Thanks to correction of E. coli genome sequence by Dr. Horiuchi, National Institute of Basic Biology, and his colleagues, we finally obtained very accurate genome sequence of this organism and more than 300 nucleotide sequences were revised. Latest E. coli genome sequence entry in public database is already applied these corrections.
Our final prediction revealed alteration of initiation or termination sites in about 760 ORFs and we assigned new id started 5000. JW_id larger than 5000 means to be something revised and you can get both previous and new id through our web page, however, in near future old information of ORF assigned new JW_id might be crossed out from our database except the link between old and new id.

(2) Release of protein localization data visualized by GFP, provided by Dr. Niki, National Institute of Genetics, Mishima.
This page was formerly protected by password for construction. Now it is open for the public. Still we will keep efforts to maintain this page especially for providing pictures using corrected GFP fusion type plasmid clones described below.

(3) Update information about Resources provided by our group. Very important information of our ASKA library (E. coli Plasmid clone library) for users.
According to the situation of genome sequence revision by Dr. HoriuchiEs group, we started to correct all of our resources based on the latest genome information. Update from 1.2 to 1.3 revealed alteration in nearly 800 ORFs. The list of target ORFs of re-construction of ASKA library can be obtained through our web site ( reclone_list.html).
If you have any plan to use ASKA clone, please check the list and if you find your target in the list, please confirm whether the clone based on old annotation is suitable for your research or wait until distribution of corrected ones.
The schedule of date for re-distribution of revised ORF clones will be until Christmas this year but just for GFP fusion type clones. GFP free type clones will be started to construct the beginning of next year. It will take about two months to be completed.
Other 27 ORF targets for ASKA library that we failed to cloned for some reason or some defects during storage, we also tried to re-construct and they will also be distributed at the same period with clones described above.

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