Systematic construction of single gene knock out mutants of all genes/ORFs including putative ones has been performed (Baba et al., 2003) by using the Wanner's method (Datsewnko and Wanner, 1997). The mutants are expected to be useful resources not only for the functional analysis of y-genes (genes of unknown functions) but also for the analysis of gene expression profiles based on DNA microarray and other methods. The collection will be termed the "Keio Collection" and will be freely distributed upon request when the construction is completed.
1) Datsenko, K.A. and Wanner, B.L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A, 97, 6640-6645.
2) Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M.,
Datsenko, K.A., Tomita, M., Wanner, B.L. and Mori, H. Construction of
Escherichia coli K-12 in-frame, single-gene knock-out mutants -- the Keio
collection. Mol Systems Biol, doi:10.1038/msb4100050 (2006).
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