Q1) How to order resource materials?

Go to request page.

Q2) What is the host strain?

BW25113 (Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97:6640-6645) for KEIO collection. AG1[recA1 endA1 gyrA96 thi-1 hsdR17(rK-mK+) supE44 relA1](commercially available from Stratagene, Inc.) for ASKA library.

Q3) Why two stocks of each deletion strain of KEIO collection?

To check suppressor mutations, independent two deletion candidates were stocked.

Q4) Possibility to distribute by other than FEDEX.

Only by FEDEX at present.

Q5) Correspondence between well position and strains.

The information is downloadable from Resource_download.html

Q6) Distribution of the host strain of KEIO collection, BW25113, from GenoBase and how?

With kind permission of Prof. Barry L. Wanner Purdue University, we accept request through the request form in our homepage and provide it.

  Resource comments and questions can be posted on the EcoliHub Forum.